kv4 3 antibodies  (Alomone Labs)

Journal: The Journal of Neuroscience

Article Title: KChIP4a is a Biophysical Amplifier of Inhibition in Atypical Dopamine Neurons and Controls Learning from Negative Prediction Errors

doi: 10.1523/JNEUROSCI.1956-25.2026

Figure Lengend Snippet: Ex3d mutation reduces KChIP4a protein expression in midbrain dopamine neurons without disrupting overall Kv4 channel subunit composition. A , Diagram schematic of the proteomic analysis of native Kv4 channel complexes. Tissue was excised from ventral midbrain and cerebellum, and Kv4 channel complexes were affinity isolated (antibodies in green, channels and associated proteins in other colors) and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) following tryptic digest. B , C , Normalized molecular abundance determined for the indicated Kv4 complex constituents from ventral midbrain (MB; B ) or from the cerebellum (CB; C ) of both CTRL and Ex3d mice (see also Fig. S1 ). Abundance was normalized to the tetrameric structure of the channel core. Note that Kv4.3 is the predominant pore-forming subunit (3/4 of each tetramer, on average), in both brain regions tested, while KChIP4 is the prevailing auxiliary subunit in MB (2/4 of each tetramer, on average), but not in CB. D , Analysis of the molecular abundance of the KChIP4a protein (relative to CTRL) based on isoform-specific peptides. Note the reduction of KChIP4a abundance in the midbrain of Ex3d mice. Data was analyzed with one-way ANOVA. N = 12 experiments for Ex3d and CTRL. Error bars indicate standard error of the mean. * p < 0.05; ** p < 0.01.

Article Snippet: Solubilizates were directly incubated with 7.5 μg Kv4.3 antibodies (Alomone Labs, #APC-017) immobilized on Protein A Dynabeads (Invitrogen) for 2 h at 4°C.

Techniques: Mutagenesis, Expressing, Isolation, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: Kv4.3 channels exhibit high expression levels in TH + neurons of DRG. (A) Representative immunofluorescence images demonstrating the colocalization of Kv4.3 (green) with neuronal markers, including TH (red), iB4 (magenta), CGRP (red), NFH (red), and parvalbumin (red) in the DRGs of wild-type mice. The results of quantitative analysis of marker coexpression are presented in a pie chart. Scale bars represent 50 μm. (B) Quantitative analysis of the percentage of Kv4.3-positive neurons that co-express each specific marker, presented as bar graphs. (C) Quantitative analysis of the percentage of neurons expressing each specific marker that are colabeled with Kv4.3, presented as bar graphs. (D) Summary data illustrating the relative fluorescence intensity of Kv4.3 in colabeled neuronal populations, with the fluorescence intensity of Kv4.3 in TH + neurons was taken as 100%. Statistical analysis performed using Mann–Whitney U test. All data presented as mean ± SEM. ∗∗∗ P < 0.001 vs. TH + neurons.

Article Snippet: The sections were permeabilized with 0.3% Triton X-100 and 3% BSA in PBS at 37 °C for 1 h. After non-specific binding was blocked with 10% goat serum for 1 h, all the sections were incubated overnight at 4 °C with primary antibodies against Kv4.3 (1:250, 75-017, NeuroMab), Piezo2 (1:200, #NBP1-78624, Novus Biologicals), Kv7.2 (1:100, PA1-929, Invitrogen), HCN1 (1:50, 55222-1-AP, Proteintech), TH (1:80, ARG11149 , Arigo), TH (1:200, 25859-1-AP, Proteintech), CGRP (1:200, ab283568, Abcam), NFH (1:200, ab207176, Abcam), and parvalbumin (1:200, 29312-1-AP, Proteintech).

Techniques: Expressing, Immunofluorescence, Marker, Fluorescence, MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: Oxaliplatin specifically downregulates Kv4.3 channel expression in TH + neurons. (A, B) Relative mRNA (A) and protein (B) expression levels of Kv4.3 and Kv4.1 in L3–L5 DRGs from control and oxaliplatin-treated mice. n = 6 per group in (A). n = 5 per group in (B). Statistical significance was determined using Student’s unpaired t test. (C) Left: Representative immunofluorescence images of Kv4.3 (green) and TH (red) in L3–L5 DRGs collected 8 days after the first dose of oxaliplatin. Scale bar: 50 μm. Right: Quantification of the relative fluorescence intensity of Kv4.3 in TH + cells. Statistical significance was determined using the Mann–Whitney U test. (D) Left: Representative immunofluorescence images of Kv4.1 (red) and TH (green) in L3–L5 DRGs collected 8 days after the first dose of oxaliplatin. Scale bar: 50 μm. Right: Quantification of relative fluorescence intensity of Kv4.1 in TH + cells. Statistical significance was determined using the Mann–Whitney U test. (E) Relative membrane protein expression level of Kv4.3 in L3–L5 DRGs from control and oxaliplatin-treated mice. n = 5 per group. Statistical significance was determined using the Mann–Whitney U test. (F) Relative nuclear protein expression level of REST in L3–L5 DRGs from control and oxaliplatin-treated mice. n = 3 per group. Statistical significance was determined using Student’s unpaired t -test. All data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. Control.

Article Snippet: The sections were permeabilized with 0.3% Triton X-100 and 3% BSA in PBS at 37 °C for 1 h. After non-specific binding was blocked with 10% goat serum for 1 h, all the sections were incubated overnight at 4 °C with primary antibodies against Kv4.3 (1:250, 75-017, NeuroMab), Piezo2 (1:200, #NBP1-78624, Novus Biologicals), Kv7.2 (1:100, PA1-929, Invitrogen), HCN1 (1:50, 55222-1-AP, Proteintech), TH (1:80, ARG11149 , Arigo), TH (1:200, 25859-1-AP, Proteintech), CGRP (1:200, ab283568, Abcam), NFH (1:200, ab207176, Abcam), and parvalbumin (1:200, 29312-1-AP, Proteintech).

Techniques: Expressing, Control, Immunofluorescence, Fluorescence, MANN-WHITNEY, Membrane

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: The pharmacological blockade of Kv4 channels potentiates mechanical allodynia. (A) Schematic representation of cannula-mediated drug administration to the DRG. (B–G) The pharmacological inhibition of Kv4.3 channels using 4-AP in the DRG significantly enhanced mechanical hypersensitivity in mice compared with vehicle-treated controls, as demonstrated by multiple behavioral assays: von Frey test (B), cotton swab test (C, D), light brush test (E, F), and sticky tape test (G) ( n = 10 per group). Statistical significance was assessed using Mann–Whitney U test. (H–L) Nociceptive responses to cold stimuli, as evaluated by the cold plate test (H), and thermal sensitivity, as assessed by the Hargreaves test (I) and hot plate test (J–L), showed no significant alterations following 4-AP administration ( n = 10 and 10 for cold plate test and Hargreaves test; n = 11 and 8 for hot plate test). Statistical analyses were performed using Mann–Whitney U test or Student’s unpaired t -test. All the data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01 vs . Vehicle.

Article Snippet: The sections were permeabilized with 0.3% Triton X-100 and 3% BSA in PBS at 37 °C for 1 h. After non-specific binding was blocked with 10% goat serum for 1 h, all the sections were incubated overnight at 4 °C with primary antibodies against Kv4.3 (1:250, 75-017, NeuroMab), Piezo2 (1:200, #NBP1-78624, Novus Biologicals), Kv7.2 (1:100, PA1-929, Invitrogen), HCN1 (1:50, 55222-1-AP, Proteintech), TH (1:80, ARG11149 , Arigo), TH (1:200, 25859-1-AP, Proteintech), CGRP (1:200, ab283568, Abcam), NFH (1:200, ab207176, Abcam), and parvalbumin (1:200, 29312-1-AP, Proteintech).

Techniques: Inhibition, MANN-WHITNEY, Hot Plate Test

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: Kv4.3 knockdown in TH + DRG neurons reduces the mechanical threshold in mice. (A) Schematic representation of localized virus injection into L3–L4 DRGs. (B, C) The knockdown efficiency of Kv4.3 in DRG neurons was validated using quantitative reverse transcription PCR (qRT-PCR; B, n = 3 per group) and Western blot analysis (C, n = 5 per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. (D–J) Kv4.3 knockdown in TH + neurons significantly enhanced pain-related behaviors in response to mechanical stimuli, as evaluated using von Frey filaments (D), cotton swabs (E, F), a light brush (G, H), and adhesive tape (I, J). n = 12 in Control, n = 13 in KD. Statistical significance was determined using Mann–Whitney U test or unpaired Student’s t -test. (K–O) Behavioral responses to noxious cold stimuli, assessed using a cold plate (K), and thermal stimuli, evaluated using the Hargreaves test (I) and hot plate tests (L–O). n = 12 in Control, n = 13 in KD. Statistical significance was determined using unpaired Student’s t -test. All data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. Control. ### P < 0.001 vs. sh-Scr.

Article Snippet: The sections were permeabilized with 0.3% Triton X-100 and 3% BSA in PBS at 37 °C for 1 h. After non-specific binding was blocked with 10% goat serum for 1 h, all the sections were incubated overnight at 4 °C with primary antibodies against Kv4.3 (1:250, 75-017, NeuroMab), Piezo2 (1:200, #NBP1-78624, Novus Biologicals), Kv7.2 (1:100, PA1-929, Invitrogen), HCN1 (1:50, 55222-1-AP, Proteintech), TH (1:80, ARG11149 , Arigo), TH (1:200, 25859-1-AP, Proteintech), CGRP (1:200, ab283568, Abcam), NFH (1:200, ab207176, Abcam), and parvalbumin (1:200, 29312-1-AP, Proteintech).

Techniques: Knockdown, Virus, Injection, Reverse Transcription, Quantitative RT-PCR, Western Blot, Adhesive, Control, MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: The pharmacological modulation of Kv4.3 channels mitigates oxaliplatin-induced neuronal hyperexcitability and mechanical allodynia. (A–G) In vitro electrophysiological recordings were performed using acutely dissociated small-diameter TH + DRG neurons from OIPN model mice. (A) Representative traces of evoked APs in TH + neurons from the control and OIPN groups. Scale bars: 10 ms and 50 mV. (B–G) Quantitative analysis of electrophysiological parameters, including rheobase (B), AP threshold (C), latency (D), RMP (E), and AP half-width (F). n = 8 cells per group. Statistical significance was assessed using paired t -tests. (G) Representative traces and quantitative analysis of APs evoked by current injection in TH + neurons. n = 8 cells per group. Statistical significance was assessed using two-way ANOVA followed by Bonferroni’s post hoc test. The data in (B–G) are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. Before. (H) Schematic of the in vivo treatment protocol: OIPN mice received intraperitoneal injections of NS5806 (2.5 mg/kg) or vehicle on Days 14–16 following initial oxaliplatin administration. (I–O) Behavioral assessments revealed that NS5806 transiently alleviated mechanical hypersensitivity, as measured by von Frey filament testing (I), and tactile allodynia, evaluated via cotton swab (J, K) and light brush tests (L, M), in a time-dependent manner. The analgesic effects peaked on the day of administration and diminished post-treatment cessation. However, no improvement was observed in the response to adhesive tape (O). n = 6 in OIPN, n = 7 in OIPN + NS5806. Statistical significance was determined using two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test. (P, Q) NS5806 did not significantly alter cold hypersensitivity (cold plate test, P) or thermal nociception (Hargreaves test, Q), as withdrawal latencies remained unchanged. n = 6 in OIPN, n = 7 in OIPN + NS5806. Statistical significance was assessed using two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test. Data in (I–Q) are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. OIPN.

Article Snippet: The sections were permeabilized with 0.3% Triton X-100 and 3% BSA in PBS at 37 °C for 1 h. After non-specific binding was blocked with 10% goat serum for 1 h, all the sections were incubated overnight at 4 °C with primary antibodies against Kv4.3 (1:250, 75-017, NeuroMab), Piezo2 (1:200, #NBP1-78624, Novus Biologicals), Kv7.2 (1:100, PA1-929, Invitrogen), HCN1 (1:50, 55222-1-AP, Proteintech), TH (1:80, ARG11149 , Arigo), TH (1:200, 25859-1-AP, Proteintech), CGRP (1:200, ab283568, Abcam), NFH (1:200, ab207176, Abcam), and parvalbumin (1:200, 29312-1-AP, Proteintech).

Techniques: In Vitro, Control, Injection, In Vivo, Adhesive

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: Kv4.3 channels exhibit high expression levels in TH + neurons of DRG. (A) Representative immunofluorescence images demonstrating the colocalization of Kv4.3 (green) with neuronal markers, including TH (red), iB4 (magenta), CGRP (red), NFH (red), and parvalbumin (red) in the DRGs of wild-type mice. The results of quantitative analysis of marker coexpression are presented in a pie chart. Scale bars represent 50 μm. (B) Quantitative analysis of the percentage of Kv4.3-positive neurons that co-express each specific marker, presented as bar graphs. (C) Quantitative analysis of the percentage of neurons expressing each specific marker that are colabeled with Kv4.3, presented as bar graphs. (D) Summary data illustrating the relative fluorescence intensity of Kv4.3 in colabeled neuronal populations, with the fluorescence intensity of Kv4.3 in TH + neurons was taken as 100%. Statistical analysis performed using Mann–Whitney U test. All data presented as mean ± SEM. ∗∗∗ P < 0.001 vs. TH + neurons.

Article Snippet: The sections were incubated with the anti-Kv4.3 primary antibody (1:250, 75-017, NeuroMab) at 4 °C overnight, followed by three times PBS washes (10 min each).

Techniques: Expressing, Immunofluorescence, Marker, Fluorescence, MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: Oxaliplatin specifically downregulates Kv4.3 channel expression in TH + neurons. (A, B) Relative mRNA (A) and protein (B) expression levels of Kv4.3 and Kv4.1 in L3–L5 DRGs from control and oxaliplatin-treated mice. n = 6 per group in (A). n = 5 per group in (B). Statistical significance was determined using Student’s unpaired t test. (C) Left: Representative immunofluorescence images of Kv4.3 (green) and TH (red) in L3–L5 DRGs collected 8 days after the first dose of oxaliplatin. Scale bar: 50 μm. Right: Quantification of the relative fluorescence intensity of Kv4.3 in TH + cells. Statistical significance was determined using the Mann–Whitney U test. (D) Left: Representative immunofluorescence images of Kv4.1 (red) and TH (green) in L3–L5 DRGs collected 8 days after the first dose of oxaliplatin. Scale bar: 50 μm. Right: Quantification of relative fluorescence intensity of Kv4.1 in TH + cells. Statistical significance was determined using the Mann–Whitney U test. (E) Relative membrane protein expression level of Kv4.3 in L3–L5 DRGs from control and oxaliplatin-treated mice. n = 5 per group. Statistical significance was determined using the Mann–Whitney U test. (F) Relative nuclear protein expression level of REST in L3–L5 DRGs from control and oxaliplatin-treated mice. n = 3 per group. Statistical significance was determined using Student’s unpaired t -test. All data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. Control.

Article Snippet: The sections were incubated with the anti-Kv4.3 primary antibody (1:250, 75-017, NeuroMab) at 4 °C overnight, followed by three times PBS washes (10 min each).

Techniques: Expressing, Control, Immunofluorescence, Fluorescence, MANN-WHITNEY, Membrane

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: The pharmacological blockade of Kv4 channels potentiates mechanical allodynia. (A) Schematic representation of cannula-mediated drug administration to the DRG. (B–G) The pharmacological inhibition of Kv4.3 channels using 4-AP in the DRG significantly enhanced mechanical hypersensitivity in mice compared with vehicle-treated controls, as demonstrated by multiple behavioral assays: von Frey test (B), cotton swab test (C, D), light brush test (E, F), and sticky tape test (G) ( n = 10 per group). Statistical significance was assessed using Mann–Whitney U test. (H–L) Nociceptive responses to cold stimuli, as evaluated by the cold plate test (H), and thermal sensitivity, as assessed by the Hargreaves test (I) and hot plate test (J–L), showed no significant alterations following 4-AP administration ( n = 10 and 10 for cold plate test and Hargreaves test; n = 11 and 8 for hot plate test). Statistical analyses were performed using Mann–Whitney U test or Student’s unpaired t -test. All the data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01 vs . Vehicle.

Article Snippet: The sections were incubated with the anti-Kv4.3 primary antibody (1:250, 75-017, NeuroMab) at 4 °C overnight, followed by three times PBS washes (10 min each).

Techniques: Inhibition, MANN-WHITNEY, Hot Plate Test

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: Kv4.3 knockdown in TH + DRG neurons reduces the mechanical threshold in mice. (A) Schematic representation of localized virus injection into L3–L4 DRGs. (B, C) The knockdown efficiency of Kv4.3 in DRG neurons was validated using quantitative reverse transcription PCR (qRT-PCR; B, n = 3 per group) and Western blot analysis (C, n = 5 per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. (D–J) Kv4.3 knockdown in TH + neurons significantly enhanced pain-related behaviors in response to mechanical stimuli, as evaluated using von Frey filaments (D), cotton swabs (E, F), a light brush (G, H), and adhesive tape (I, J). n = 12 in Control, n = 13 in KD. Statistical significance was determined using Mann–Whitney U test or unpaired Student’s t -test. (K–O) Behavioral responses to noxious cold stimuli, assessed using a cold plate (K), and thermal stimuli, evaluated using the Hargreaves test (I) and hot plate tests (L–O). n = 12 in Control, n = 13 in KD. Statistical significance was determined using unpaired Student’s t -test. All data are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. Control. ### P < 0.001 vs. sh-Scr.

Article Snippet: The sections were incubated with the anti-Kv4.3 primary antibody (1:250, 75-017, NeuroMab) at 4 °C overnight, followed by three times PBS washes (10 min each).

Techniques: Knockdown, Virus, Injection, Reverse Transcription, Quantitative RT-PCR, Western Blot, Adhesive, Control, MANN-WHITNEY

Journal: Acta Pharmaceutica Sinica. B

Article Title: The Kv4.3 channel on c-low threshold mechanoreceptors in DRG is a key mediator transforming tactile stimuli into nociceptive signals in chemotherapy-induced neuropathy

doi: 10.1016/j.apsb.2025.12.005

Figure Lengend Snippet: The pharmacological modulation of Kv4.3 channels mitigates oxaliplatin-induced neuronal hyperexcitability and mechanical allodynia. (A–G) In vitro electrophysiological recordings were performed using acutely dissociated small-diameter TH + DRG neurons from OIPN model mice. (A) Representative traces of evoked APs in TH + neurons from the control and OIPN groups. Scale bars: 10 ms and 50 mV. (B–G) Quantitative analysis of electrophysiological parameters, including rheobase (B), AP threshold (C), latency (D), RMP (E), and AP half-width (F). n = 8 cells per group. Statistical significance was assessed using paired t -tests. (G) Representative traces and quantitative analysis of APs evoked by current injection in TH + neurons. n = 8 cells per group. Statistical significance was assessed using two-way ANOVA followed by Bonferroni’s post hoc test. The data in (B–G) are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. Before. (H) Schematic of the in vivo treatment protocol: OIPN mice received intraperitoneal injections of NS5806 (2.5 mg/kg) or vehicle on Days 14–16 following initial oxaliplatin administration. (I–O) Behavioral assessments revealed that NS5806 transiently alleviated mechanical hypersensitivity, as measured by von Frey filament testing (I), and tactile allodynia, evaluated via cotton swab (J, K) and light brush tests (L, M), in a time-dependent manner. The analgesic effects peaked on the day of administration and diminished post-treatment cessation. However, no improvement was observed in the response to adhesive tape (O). n = 6 in OIPN, n = 7 in OIPN + NS5806. Statistical significance was determined using two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test. (P, Q) NS5806 did not significantly alter cold hypersensitivity (cold plate test, P) or thermal nociception (Hargreaves test, Q), as withdrawal latencies remained unchanged. n = 6 in OIPN, n = 7 in OIPN + NS5806. Statistical significance was assessed using two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test. Data in (I–Q) are presented as mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. OIPN.

Article Snippet: The sections were incubated with the anti-Kv4.3 primary antibody (1:250, 75-017, NeuroMab) at 4 °C overnight, followed by three times PBS washes (10 min each).

Techniques: In Vitro, Control, Injection, In Vivo, Adhesive